Enhancement of the Production of SAM by Overexpression
of SAM Synthetase in Pichia pastoris
YU Zhi-Liang2, WU Xing-Jia, LI Dong-Yang, YANG Sheng1, ZHOU Zheng, CAI Jin2, YUAN Zhong-Yi*
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological
Sciences,
the Chinese Academy of Sciences, Shanghai 200031, China;
1 Institute of Plant Physiology and Ecology,
Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032, China;
2 College of Material and Chemical Engineering, Zhejiang University, Hangzhou 310027, China )
Abstract
S-Adenosyl-L-methionine(SAM) is an important metabolic intermediate in the metabolic flux of sulphur. SAM is involved in three key metabolic pathways: transmethylation, transsulfuration and polyamine synthesis. As a potential therapeutic agent, SAM is being used as over the counter drug and nutrient supplement. An expression vector, harboring SAM synthetase 2 gene from S. cerevisiae and regulated by the glyceraldehyde-3-phosphate dehydrogenase gene promoter P
Key words S-Adenosyl-L-methionine; SAM synthetase 2; P. pastoris; metabolic eng ineering; expression optimization
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